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Anti- PG II Antibody (
Datasheet)
| Source | Mouse monoclonal antibody，cultured in vitro | ||
| Platforms | Immunofluorescence, Chemiluminescence, Colloidal gold and Immunological latex turbidimetry | ||
| Catalog number | K28c9 | K30t2 | K101h3 |
| Applications | Capture | Detection | Capture |
| Buffer | 1x PBS | ||
| Purity | Purity>98%，purified by Protein A/G chromatography | ||
| Storage instructions | Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. | ||
PG II protein ( Datasheet)
| Description | PG II protein (41.4kD) with His-tag, cultured in vitro |
| Applications | Calibrator and quality control product |
| Catalog number | K3926 |
| Source | Mammalian cell |
| Purity | >90% as analysized by SDS-PAGE |
| Buffer | 1 x PBS，pH 7.4 |
| Storage instructions | Store at 2 - 8°C (35.6 – 46.4°F) for up to 3 years. |
| Sequence | AVVKVPLKKF KSIRETMKEK GLLGEFLRTH KYDPAWKYRF GDLSVTYEPM AYMDAAYFGE ISIGTPPQNF LVLFDTGSSN LWVPSVYCQS QACTSHSRFN PSESSTYSTN GQTFSLQYGS GSLTGFFGYD TLTVQSIQVP NQEFGLSENE PGTNFVYAQF DGIMGLAYPA LSVDEATTAM QGMVQEGALT SPVFSVYLSN QQGSSGGAVV FGGVDSSLYT GQIYWAPVTQ ELYWQIGIEE FLIGGQASGW CSEGCQAIVD TGTSLLTVPQ QYMSALLQAT GAQEDEYGQF LVNCNSIQNL PSLTFIINGV EFPLPPSSYI LSNNGYCTVG VEPTYLSSQN GQPLWILGDV FLRSYYSVYD LGNNRVGFAT AAHHHHHH |
| SDS-PAGE |  |
Antibody Product Information
Fig. 1 Clinical sample analysis| Sample (ng/ml) |
Test 1 | Test 2 | Test 3 | Average (ng/ml) |
RSD | Standard | |
| Batch 1 | 17.32 | 17.32 | 16.98 | 18.34 | 17.55 | 1.31% | ±15% |
| 49.56 | 48.39 | 44.82 | 49.31 | 47.51 | -4.14% | ||
| Batch 2 | 17.32 | 16.23 | 15.75 | 17.36 | 16.45 | -5.04% | |
| 49.56 | 49.33 | 47.6 | 48.70 | 48.54 | -2.05% | ||
| Batch 3 | 17.32 | 17.22 | 16.71 | 16.02 | 16.65 | -3.87% | |
| 49.56 | 47.71 | 51.41 | 51.75 | 50.29 | 1.47% |
) ×100% (CV < 15% as indicated in the following table)| Low Value （17.32ng/ml） |
High Value （49.56ng/ml） |
||
| Test 1 | 18.83 | 50.60 | |
| Test 2 | 16.63 | 48.22 | |
| Test 3 | 18.62 | 44.81 | |
| Test 4 | 18.46 | 53.38 | |
| Test 5 | 17.25 | 50.75 | |
| Test 6 | 15.73 | 51.48 | |
| Test 7 | 18.24 | 52.40 | |
| Test 8 | 18.25 | 49.70 | |
| Test 9 | 18.54 | 45.39 | |
| Test 10 | 15.87 | 45.43 | |
| Average(ng/ml) | 17.64 | 49.22 | |
| SD | 1.18 | 3.10 | |
| CV | 6.69% | 6.30% | |
| Standard | ±15% | ||
Fig. 2 Calibration curve| Theoretical value (ng/ml) | Measured value 1 | Measured value 2 | Average |
|---|---|---|---|
| 0.00 | 2.1 | 1.54 | 1.82 |
| 12.50 | 11.22 | 12.02 | 11.62 |
| 25.00 | 25.8 | 24.8 | 25.3 |
| 50.00 | 48.25 | 47.36 | 47.805 |
| 100.00 | 98.76 | 96.74 | 97.75 |
Fig. 3 Linearity range
Fig. 4 Calibration curve
Description
