NT-proB-type Natriuretic Peptide (NT-proBNP)

Antibody Evaluation (Colloidal Gold-based)

Colloidal gold-based immunochromatographic test strip is prepared with K23d4-K16q7 (30 Roche calibrators, Concentration: 0.05-30 ng/ml, R² >98%)

Antibody Evaluation (Immunofluorescence-based)
Immunofluorescence-Based test strip is prepared with K23d4-K16q7 (30 Roche calibrators, Concentration: 0.05-30 ng/ml, R² >98%)

Accuracy
The quality control samples with concentration of 2000pg/ml-10000pg/ml and 200pg/ml-800pg/ml respectively were required to repeat test three times. B= (M-T) / T×100% (B: Relative Standard Deviation, M: Average, T: Concentration)

Repeatability
The quality control samples with concentration of 2000pg/ml-10000pg/ml and 200pg/ml-800pg/ml respectively were required to repeat test over 10 timers. CV= (S /) ×100% (CV < 15% as indicated in the following table)

B-type natriuretic peptide (brain natriuretic peptide: BNP) is a small, ringed peptide secreted by the heart to regulate blood pressure and fluid balance. The peptide is stored in and secreted predominantly from membrane granules in the heart ventricles in a pro form (preproBNP). The prohormone is clevaged into preBNP, then processed to a biologically active form BNP with 32 amino acids, and another biologically inactive form N-terminal proBNP (NT-proBNP) with 76 amino acids. NT-proBNP is with a relatively long plasma half-life, which would lead to a higher stability of its levels in patients. NT-proBNP is emerged as biomarkers of heart failure (HF) in clinical studies.

NT-proBNP is an independent predictor of coronary artery disease (CAD) such as acute coronary syndrome (ACS) and stable angina pectoris (SAP). A significant correlation was observed between BNP and NT-proBNP at all stages of kidney function, but the correlation was significantly affected by kidney function. Observational studies showed a close inverse relationship between plasma NT-proBNP and creatinine clearance. NT-proBNP is also increased in left ventricular hypertrophy and left ventricular systolic and diastolic dysfunction. It is also indicated that an age- and gender difference in the ability of NT-proBNP is considerate to identify myocardial dysfunction in relation to metabolic disturbances.